Our first run with the OpenPCR machine just kicked off. We’re amplifying a SNP for bitter tasting. 2 samples each from Josh, Xia, and myself. One was made using a plastic swab against the inside of our cheek, the other made by putting a piece of Kim wipe against our cheek.
Kicked it off at 10:10 pm, 30 cycles to go, 94, 68, 84!
To follow up on this, the OpenPCR machine looked to perform well. However, we weren’t able to see any bands in our gel — including our ladder, which indicates that something went wrong in our gel. Unfortunately, that means we can’t confirm how well this prototype of the OpenPCR machine functions. For next time!