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	<title>Comments for OpenPCR - Open source, hackable, personal PCR machine</title>
	<atom:link href="http://openpcr.org/comments/feed/" rel="self" type="application/rss+xml" />
	<link>http://openpcr.org</link>
	<description>DNA amplification for everyone</description>
	<lastBuildDate>Sun, 15 Jan 2012 00:03:34 +0000</lastBuildDate>
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		<title>Comment on Eri Gentry &#8211; Open Science Conference (OpenPCR cameo!) by mike verbeeck</title>
		<link>http://openpcr.org/2011/07/eri-gentry-open-science-conference-openpcr-cameo/comment-page-1/#comment-19883</link>
		<dc:creator>mike verbeeck</dc:creator>
		<pubDate>Sun, 15 Jan 2012 00:03:34 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=951#comment-19883</guid>
		<description>Hello,

We have alread the tools for doing PCR on our own but still depend on secuencing facilities to provide us with the results. How does the things for an openSequencing machine?  How long will we have to look into the future for this considering present technology?

thanks
mikev</description>
		<content:encoded><![CDATA[<p>Hello,</p>
<p>We have alread the tools for doing PCR on our own but still depend on secuencing facilities to provide us with the results. How does the things for an openSequencing machine?  How long will we have to look into the future for this considering present technology?</p>
<p>thanks<br />
mikev</p>
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	<item>
		<title>Comment on PCR Machine Patents are Dead by Sampson</title>
		<link>http://openpcr.org/2010/06/pcr-patents-are-dead/comment-page-1/#comment-18166</link>
		<dc:creator>Sampson</dc:creator>
		<pubDate>Mon, 14 Nov 2011 21:04:03 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=222#comment-18166</guid>
		<description>http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&amp;Sect2=HITOFF&amp;d=PALL&amp;p=1&amp;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&amp;r=1&amp;f=G&amp;l=50&amp;s1=7074367.PN.&amp;OS=PN/7074367&amp;RS=PN/7074367

http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&amp;Sect2=HITOFF&amp;d=PALL&amp;p=1&amp;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&amp;r=1&amp;f=G&amp;l=50&amp;s1=6767512.PN.&amp;OS=PN/6767512&amp;RS=PN/6767512

gradient PCR seems like it is still 4+ years away from expiring - albeit you can use other techniques to generate a temperature gradient (perhaps more than one block) although it might not be cost-effective!

Personally I plan on modifying OpenPCR to a gradient PCR.

Best of luck!</description>
		<content:encoded><![CDATA[<p><a href="http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&#038;Sect2=HITOFF&#038;d=PALL&#038;p=1&#038;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&#038;r=1&#038;f=G&#038;l=50&#038;s1=7074367.PN.&#038;OS=PN/7074367&#038;RS=PN/7074367" rel="nofollow">http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&#038;Sect2=HITOFF&#038;d=PALL&#038;p=1&#038;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&#038;r=1&#038;f=G&#038;l=50&#038;s1=7074367.PN.&#038;OS=PN/7074367&#038;RS=PN/7074367</a></p>
<p><a href="http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&#038;Sect2=HITOFF&#038;d=PALL&#038;p=1&#038;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&#038;r=1&#038;f=G&#038;l=50&#038;s1=6767512.PN.&#038;OS=PN/6767512&#038;RS=PN/6767512" rel="nofollow">http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&#038;Sect2=HITOFF&#038;d=PALL&#038;p=1&#038;u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&#038;r=1&#038;f=G&#038;l=50&#038;s1=6767512.PN.&#038;OS=PN/6767512&#038;RS=PN/6767512</a></p>
<p>gradient PCR seems like it is still 4+ years away from expiring &#8211; albeit you can use other techniques to generate a temperature gradient (perhaps more than one block) although it might not be cost-effective!</p>
<p>Personally I plan on modifying OpenPCR to a gradient PCR.</p>
<p>Best of luck!</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by DNA is now DIY: OpenPCR ships worldwide &#124; OpenPCR &#8211; Open source, hackable PCR machine &#124; CT&#039;s Blog</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-18055</link>
		<dc:creator>DNA is now DIY: OpenPCR ships worldwide &#124; OpenPCR &#8211; Open source, hackable PCR machine &#124; CT&#039;s Blog</dc:creator>
		<pubDate>Thu, 10 Nov 2011 09:44:58 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-18055</guid>
		<description>[...] DNA is now DIY: OpenPCR ships worldwide &#124; OpenPCR &#8211; Open source, hackable PCR machine. [...]</description>
		<content:encoded><![CDATA[<p>[...] DNA is now DIY: OpenPCR ships worldwide | OpenPCR &#8211; Open source, hackable PCR machine. [...]</p>
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	<item>
		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Open Source DNA-Kopierer &#124; cyberhive.info</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-17501</link>
		<dc:creator>Open Source DNA-Kopierer &#124; cyberhive.info</dc:creator>
		<pubDate>Tue, 18 Oct 2011 17:49:36 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-17501</guid>
		<description>[...] über 12000 Dollar einsammelten. Heute verbreitete sich bei Twitter die Nachricht, dass die Geräte zum weltweiten Versand freigegeben [...]</description>
		<content:encoded><![CDATA[<p>[...] über 12000 Dollar einsammelten. Heute verbreitete sich bei Twitter die Nachricht, dass die Geräte zum weltweiten Versand freigegeben [...]</p>
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	</item>
	<item>
		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Open PCR: DNA is now DIY - Nerdcore</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-17425</link>
		<dc:creator>Open PCR: DNA is now DIY - Nerdcore</dc:creator>
		<pubDate>Sat, 15 Oct 2011 17:39:42 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-17425</guid>
		<description>[...] knapp über 500$ gibts jetzt das OpenPCR-Kit, mit dem Nerd zu Hause DNA untersuchen kann. Das Teil hat eine USB-Schnittstelle und ist nicht nur [...]</description>
		<content:encoded><![CDATA[<p>[...] knapp über 500$ gibts jetzt das OpenPCR-Kit, mit dem Nerd zu Hause DNA untersuchen kann. Das Teil hat eine USB-Schnittstelle und ist nicht nur [...]</p>
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	</item>
	<item>
		<title>Comment on Eri Gentry &#8211; Open Science Conference (OpenPCR cameo!) by wangqin</title>
		<link>http://openpcr.org/2011/07/eri-gentry-open-science-conference-openpcr-cameo/comment-page-1/#comment-15946</link>
		<dc:creator>wangqin</dc:creator>
		<pubDate>Tue, 16 Aug 2011 03:11:27 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=951#comment-15946</guid>
		<description>Hi,I&#039;m wangqin,from china.Now, I&#039;m working for Educational Equipment Research Institute of Ministry of Education.OpenPCR is a intersting inovation,as I  found it on net, I was attracted to it immediately.  It is a good idea! it is so convient that  Using OpenPCR, you can do PCR experiment everywhere in a low costs. To my surprise, it can be driven only by a small notebook. I wonder may be any other device with a usb port , such as  a iPad or  somethin  else, can drive  OpenPCRkit in regular enviroment and in wild.  
Maybe there have  some opptunities to us to improve OPenPcr kit and the idea of OpenScience in china.</description>
		<content:encoded><![CDATA[<p>Hi,I&#8217;m wangqin,from china.Now, I&#8217;m working for Educational Equipment Research Institute of Ministry of Education.OpenPCR is a intersting inovation,as I  found it on net, I was attracted to it immediately.  It is a good idea! it is so convient that  Using OpenPCR, you can do PCR experiment everywhere in a low costs. To my surprise, it can be driven only by a small notebook. I wonder may be any other device with a usb port , such as  a iPad or  somethin  else, can drive  OpenPCRkit in regular enviroment and in wild.<br />
Maybe there have  some opptunities to us to improve OPenPcr kit and the idea of OpenScience in china.</p>
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	</item>
	<item>
		<title>Comment on DNA Fingerprinting Osama Bin Laden by Human Fingerprinting Pcr Lab</title>
		<link>http://openpcr.org/2011/05/dna-fingerprinting-osama-bin-laden/comment-page-1/#comment-15933</link>
		<dc:creator>Human Fingerprinting Pcr Lab</dc:creator>
		<pubDate>Mon, 15 Aug 2011 16:28:36 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=658#comment-15933</guid>
		<description>[...] DNA Fingerprinting Osama Bin Laden Fingerprinting begins with a DNA sample. A quick and easy source of DNA is spit. Spit has cheek cells in it and every cheek cell has a copy of the entire human genome. The human genome is 3 billion pieces of information. As comparison, a Tweet contains up to 140   Next, you&#039;ll need a PCR machine such as OpenPCR, which is like a xerox machine for DNA. Starting with a small DNA sample such as a spit sample, a PCR machine will selectively copy just the 13 STR sequences. [...]</description>
		<content:encoded><![CDATA[<p>[...] DNA Fingerprinting Osama Bin Laden Fingerprinting begins with a DNA sample. A quick and easy source of DNA is spit. Spit has cheek cells in it and every cheek cell has a copy of the entire human genome. The human genome is 3 billion pieces of information. As comparison, a Tweet contains up to 140   Next, you&#039;ll need a PCR machine such as OpenPCR, which is like a xerox machine for DNA. Starting with a small DNA sample such as a spit sample, a PCR machine will selectively copy just the 13 STR sequences. [...]</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Interzona</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15769</link>
		<dc:creator>Interzona</dc:creator>
		<pubDate>Tue, 09 Aug 2011 16:40:27 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15769</guid>
		<description>Hi its great to see affordable acces to this kind of technology. It would be good to see a cheap  supply of Polymerase for DIY experimentalists. Also Someone mentioned incorporating an LED light and detector to allow the machine to do qPCR experiments. That would be really cool for around the £500 dollar mark. 

Bravo</description>
		<content:encoded><![CDATA[<p>Hi its great to see affordable acces to this kind of technology. It would be good to see a cheap  supply of Polymerase for DIY experimentalists. Also Someone mentioned incorporating an LED light and detector to allow the machine to do qPCR experiments. That would be really cool for around the £500 dollar mark. </p>
<p>Bravo</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Moneyfox</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15452</link>
		<dc:creator>Moneyfox</dc:creator>
		<pubDate>Fri, 29 Jul 2011 07:15:34 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15452</guid>
		<description>Awsome!!
This is exactly what I wanted!
Hope bio-hacking will be popular in near future.</description>
		<content:encoded><![CDATA[<p>Awsome!!<br />
This is exactly what I wanted!<br />
Hope bio-hacking will be popular in near future.</p>
]]></content:encoded>
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		<title>Comment on OpenPCR in the Wild + We need your help! by OpenPCR in the Wild! &#124; K-Mart</title>
		<link>http://openpcr.org/2011/07/openpcr-in-the-wild-we-need-your-help/comment-page-1/#comment-15405</link>
		<dc:creator>OpenPCR in the Wild! &#124; K-Mart</dc:creator>
		<pubDate>Wed, 27 Jul 2011 15:41:56 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=901#comment-15405</guid>
		<description>[...] the OpenPCR Blog  Bio: MAKE citizen science author Tito Jankowski works on making biotech easier to do, including [...]</description>
		<content:encoded><![CDATA[<p>[...] the OpenPCR Blog  Bio: MAKE citizen science author Tito Jankowski works on making biotech easier to do, including [...]</p>
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	<item>
		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Electronics-Lab.com Blog &#187; Blog Archive &#187; DNA is now DIY: OpenPCR ships worldwide</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15372</link>
		<dc:creator>Electronics-Lab.com Blog &#187; Blog Archive &#187; DNA is now DIY: OpenPCR ships worldwide</dc:creator>
		<pubDate>Mon, 25 Jul 2011 19:54:36 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15372</guid>
		<description>[...] is now DIY: OpenPCR ships worldwide - [Link]   Tags: DNA, OpenPCR, PCR   Filed in Miscellaneous  &#124; 1 views  No Comments [...]</description>
		<content:encoded><![CDATA[<p>[...] is now DIY: OpenPCR ships worldwide &#8211; [Link]   Tags: DNA, OpenPCR, PCR   Filed in Miscellaneous  | 1 views  No Comments [...]</p>
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	<item>
		<title>Comment on It Lives by Vladimir</title>
		<link>http://openpcr.org/2011/01/it-lives/comment-page-1/#comment-15337</link>
		<dc:creator>Vladimir</dc:creator>
		<pubDate>Sat, 23 Jul 2011 14:23:26 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=492#comment-15337</guid>
		<description>In addition to the PCR machine what else is required to have a basic DNA lab? If I am not mistaken, PCR creates enough material for the analysis but does not do any analysis by itself....

Thank you!</description>
		<content:encoded><![CDATA[<p>In addition to the PCR machine what else is required to have a basic DNA lab? If I am not mistaken, PCR creates enough material for the analysis but does not do any analysis by itself&#8230;.</p>
<p>Thank you!</p>
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		<title>Comment on Putting the Open in OpenPCR by blinko</title>
		<link>http://openpcr.org/2011/05/putting-the-open-in-openpcr/comment-page-1/#comment-15286</link>
		<dc:creator>blinko</dc:creator>
		<pubDate>Thu, 21 Jul 2011 15:04:10 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=772#comment-15286</guid>
		<description>Please refer to this list if you&#039;re having trouble formulating your own open source license:  
http://www.opensource.org/licenses
It would be good to know that you&#039;re not just selling a DIY kit or low-cost PCR, and that it is actually an open product as the name implies.</description>
		<content:encoded><![CDATA[<p>Please refer to this list if you&#8217;re having trouble formulating your own open source license:<br />
<a href="http://www.opensource.org/licenses" rel="nofollow">http://www.opensource.org/licenses</a><br />
It would be good to know that you&#8217;re not just selling a DIY kit or low-cost PCR, and that it is actually an open product as the name implies.</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by OpenPCR Shows How Design And Manufacturing Are Changing &#124; BeeHiveTech.com</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15181</link>
		<dc:creator>OpenPCR Shows How Design And Manufacturing Are Changing &#124; BeeHiveTech.com</dc:creator>
		<pubDate>Mon, 18 Jul 2011 12:03:23 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15181</guid>
		<description>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</description>
		<content:encoded><![CDATA[<p>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by OpenPCR Shows How Design And Manufacturing Are Changing</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15110</link>
		<dc:creator>OpenPCR Shows How Design And Manufacturing Are Changing</dc:creator>
		<pubDate>Fri, 15 Jul 2011 14:08:28 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15110</guid>
		<description>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</description>
		<content:encoded><![CDATA[<p>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</p>
]]></content:encoded>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by ????????????? ??? ??????? hardware ??? ???? ????????&#8230; &#171; elkosmas.gr</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15102</link>
		<dc:creator>????????????? ??? ??????? hardware ??? ???? ????????&#8230; &#171; elkosmas.gr</dc:creator>
		<pubDate>Fri, 15 Jul 2011 09:08:48 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15102</guid>
		<description>[...] ?????? ?? OpenPCR ?????????? ?? ????? ? ????? ???????? ??? ????? ??????? ??????????? ??????? ?? ???? ?????? ???? (??????????? ???????? ????????? [...]</description>
		<content:encoded><![CDATA[<p>[...] ?????? ?? OpenPCR ?????????? ?? ????? ? ????? ???????? ??? ????? ??????? ??????????? ??????? ?? ???? ?????? ???? (??????????? ???????? ????????? [...]</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by OpenPCR Shows How Design And Manufacturing Are Changing &#171; Cool Green Magazine</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15080</link>
		<dc:creator>OpenPCR Shows How Design And Manufacturing Are Changing &#171; Cool Green Magazine</dc:creator>
		<pubDate>Thu, 14 Jul 2011 16:57:00 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15080</guid>
		<description>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</description>
		<content:encoded><![CDATA[<p>[...] amazing demonstration of where technology is going, which is everywhere, including your living room. The developers write: So, do people in garages have great PCR machines? Not really. Howabout high school or middle school [...]</p>
]]></content:encoded>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Sket på nettet den 14.07.11</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15067</link>
		<dc:creator>Sket på nettet den 14.07.11</dc:creator>
		<pubDate>Thu, 14 Jul 2011 07:15:36 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15067</guid>
		<description>[...] DNA is now DIY: OpenPCR ships worldwide &#124; OpenPCR &#8211; Open source, hackable PCR machine &#8212; 9:33pm via [...]</description>
		<content:encoded><![CDATA[<p>[...] DNA is now DIY: OpenPCR ships worldwide | OpenPCR &#8211; Open source, hackable PCR machine &mdash; 9:33pm via [...]</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by DNA is now DIY: OpenPCR ships worldwide &#124; dev.SquareCows.com</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15059</link>
		<dc:creator>DNA is now DIY: OpenPCR ships worldwide &#124; dev.SquareCows.com</dc:creator>
		<pubDate>Wed, 13 Jul 2011 22:00:21 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15059</guid>
		<description>[...] Visit the OpenPCR blog for the full story! [...]</description>
		<content:encoded><![CDATA[<p>[...] Visit the OpenPCR blog for the full story! [...]</p>
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		<title>Comment on DNA is now DIY: OpenPCR ships worldwide by Jenn</title>
		<link>http://openpcr.org/2011/07/dna-is-now-diy-openpcr-ships-worldwide/comment-page-1/#comment-15042</link>
		<dc:creator>Jenn</dc:creator>
		<pubDate>Wed, 13 Jul 2011 01:00:21 +0000</pubDate>
		<guid isPermaLink="false">http://openpcr.org/?p=829#comment-15042</guid>
		<description>Can you post more detailed information on what is actually included in the kit and what is not (e.g. polymerase, oligos)?  Seems unlikely that companies that sell to laboratories would sell to the home user ...

Do you also have Southern blots that confirm presence of intended DNA by restriction endonucleases?  In other words, what is the replicability of this new machine versus the old one?

If this is legit ... incredible idea.  Can&#039;t wait to see what is done with it.</description>
		<content:encoded><![CDATA[<p>Can you post more detailed information on what is actually included in the kit and what is not (e.g. polymerase, oligos)?  Seems unlikely that companies that sell to laboratories would sell to the home user &#8230;</p>
<p>Do you also have Southern blots that confirm presence of intended DNA by restriction endonucleases?  In other words, what is the replicability of this new machine versus the old one?</p>
<p>If this is legit &#8230; incredible idea.  Can&#8217;t wait to see what is done with it.</p>
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